PARL Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9H300 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 8C4B2; 1H12E10 |
Isotype | IgG2b |
Calculated MW | 42190 Da |
Description | PARL: presenilin associated, rhomboid-like. This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Immunogen | Purified recombinant fragment of PARL (aa112-167) expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Gene ID | 55486 |
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Other Names | Presenilins-associated rhomboid-like protein, mitochondrial, 3.4.21.105, Mitochondrial intramembrane cleaving protease PARL, P-beta, Pbeta, PARL, PSARL |
Dilution | WB~~1/500 - 1/2000 IHC~~1:200~~1000 IF~~1:200~1000. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PARL Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PARL |
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Synonyms | PSARL |
Function | Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals (By similarity). Required for the maturation of PINK1 into its 52kDa mature form after its cleavage by mitochondrial-processing peptidase (MPP) (PubMed:22354088). Promotes cleavage of serine/threonine-protein phosphatase PGAM5 in damaged mitochondria in response to loss of mitochondrial membrane potential (PubMed:22915595). Mediates differential cleavage of PINK1 and PGAM5 depending on the health status of mitochondria, disassociating from PINK1 and associating with PGAM5 in response to mitochondrial membrane potential loss (PubMed:22915595). Required for processing of CLPB into a form with higher protein disaggregase activity by removing an autoinhibitory N-terminal peptide (PubMed:28288130, PubMed:32573439). Promotes processing of DIABLO/SMAC in the mitochondrion which is required for DIABLO apoptotic activity (PubMed:28288130). Also required for cleavage of STARD7 and TTC19 (PubMed:28288130). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain (PubMed:14732705, PubMed:17116872). |
Cellular Location | Mitochondrion inner membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
References
1. J Alzheimers Dis. 2001 Apr;3(2):181-190. 2. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. 3. Nature. 2003 May 29;423(6939):537-41.
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