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HDAC3 Antibody

Mouse Monoclonal Antiobody to HDAC3

  • WB - HDAC3 Antibody AO1206b
    Figure 1: Western blot analysis using HDAC3 mouse mAb against truncated Trx-HDAC3 recombinant protein (1), full length HDAC3-hIgGFc (aa1-428) transfected CHO-K1 cell lysate(2) and Hela cell lysate (3).
  • IHC - HDAC3 Antibody AO1206b
    Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus cancer (left) and breast carcinoma tissue (right), showing nuclear localization with DAB staining using HDAC3 mouse mAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15379
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A7B5
Isotype Mouse IgG1
Calculated MW 48848 Da
Description HDAC3: histone deacetylase 3, also known as HD3, RPD3, RPD3-2. Entrez Protein NC_000005. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
Immunogen Purified recombinant fragment of HDAC3(aa224-428) expressed in E. Coli.
Additional Information
Gene ID 8841
Other Names Histone deacetylase 3, HD3, RPD3-2, SMAP45, HDAC3
Target/Specificity Purified recombinant fragment of HDAC3(aa224-428) expressed in E. Coli.
Dilution WB~~1:500~~2000
Format Ascitic fluid containing 0.03% sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHDAC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HDAC3
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys- 27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).
Cellular Location Nucleus. Cytoplasm. Note=Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803).
Tissue Location Widely expressed.
Research Areas
Citations ( 0 )


1. Folia Biol (Praha). 2006;52(1-2):21-33.

2. Mol Cell Biol. 2007 Feb;27(4):1280-95.

3. Mol Carcinog. 2008 Feb;47(2):137-47.

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$ 385.00
Cat# AO1206b
(40 western blots)
Availability: 7-10 days
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