- CITATIONS: 5
|Application ||WB, E|
|Calculated MW||39825 Da|
|Description||CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.|
|Immunogen||Purified recombinant fragment of CD33 (48-258) expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Monocytic/myeloid lineage cells.|
Provided below are standard protocols that you may find useful for product applications.
1. Exp Hematol. 2005 Feb;33(2):199-211. 2. Cancer. 2008 Feb 1;112(3):572-80.
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