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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD33 Antibody   

CD33 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CD33 Antibody AO1215a
    Figure 1: Western blot analysis using CD33 mouse mAb against truncated Trx-CD33 recombinant protein (1),truncated CD33 (aa48-258)-His recombinant protein (2) and truncated CD33 (aa18-259)-hIgGFc transfected CHO-K1 cell lysate (3).
  • FC - CD33 Antibody AO1215a
    Figure 3: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
  • ICC - CD33 Antibody AO1215a
    Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
  • IF - CD33 Antibody AO1215a
    Figure 3: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-beta Actin mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20138
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2B7C12
Isotype IgG1
Calculated MW 39825 Da
Description CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Immunogen Purified recombinant fragment of CD33 (48-258) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 945
Other Names Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD33
Synonyms SIGLEC3
Function Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Monocytic/myeloid lineage cells.
Research Areas
Citations ( 0 )


1. Exp Hematol. 2005 Feb;33(2):199-211. 2. Cancer. 2008 Feb 1;112(3):572-80.

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$ 385.00
Cat# AO1215a
(40 western blots)
Availability: 7-10 days
Bulk Size
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