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beta-Actin AntibodyMouse Monoclonal Antibody to beta-Actin
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AO1215a | 0.1ml (1.0mg/ml) 400 ul | 7-10 days | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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- Backgrounds
beta-Actin Antibody - Product info | |
| Application | WB, IF, FC
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| Primary Accession | P60709 |
| Other Accession | NP_001092.1 |
| Reactivity | Human, Hamster, Mouse, Monkey, Rat |
| Isotype | Mouse IgG2b |
| Clone Names | 8H10D10 |
| Calculated MW | 41737 Da |
beta-Actin Antibody - Additional info | |
| Gene ID 60 | |
| Other Names PS1TP5BP1; ACTB | |
| Target/Specificity Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH. | |
| Dilution WB~~1:500 - 2000. IF~~1:200 - 1000. FC~~1:200 - 400. | |
| Format Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions beta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
beta-Actin Antibody - Protein Information | |
| Name ACTB | |
| Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells | |
| Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs | |
beta-Actin Antibody - Related products
AM1021a: Beta-actin Antibody (Ascites)
beta-Actin Antibody - Application data
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Figure 1: Western blot analysis using anti beta-Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12(5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
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Figure 3: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-beta Actin mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
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Figure 4: Flow cytometric analysis of MCF-7 cells using anti-beta Actin mAb (right) and negative control (left).
beta-Actin Antibody - Research Areas
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BACKGROUND
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
REFERENCES
1. Proteomics. 2005 Oct;5(15):3876-84.
2. PLoS Med. 2005 Oct;2(10):e263.
3. Mol Biol Cell. 2005 Nov;16(11):5055-60
4. Nature. 2005 Oct 20;437(7062):1173-8.
