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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   beta-Actin Antibody   

beta-Actin Antibody

Purified Mouse Monoclonal Antibody

  • WB - beta-Actin Antibody AO1221a
    Figure 1: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
  • IF - beta-Actin Antibody AO1221a
    Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
  • FC - beta-Actin Antibody AO1221a
    Figure 3: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
  • IHC - beta-Actin Antibody AO1221a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 mouse mAb.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60709
Reactivity Human, Mouse, Rat, Hamster, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 8H10D10
Isotype IgG2b
Calculated MW 42kDa
Description Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Immunogen Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
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1. Proteomics. 2005 Oct;5(15):3876-84. 2. PLoS Med. 2005 Oct;2(10):e263. 3. Mol Biol Cell. 2005 Nov;16(11):5055-60 4. Nature. 2005 Oct 20;437(7062):1173-8.

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$ 325.00
Cat# AO1221a
(40 western blots)
Availability: 7-10 days
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