|Application ||WB, FC, ICC, E|
|Reactivity||Human, Mouse, Rat, Hamster, Monkey|
|Description||Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.|
|Immunogen||Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||beta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.|
|Cellular Location||Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs|
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Provided below are standard protocols that you may find useful for product applications.
1. Proteomics. 2005 Oct;5(15):3876-84. 2. PLoS Med. 2005 Oct;2(10):e263. 3. Mol Biol Cell. 2005 Nov;16(11):5055-60 4. Nature. 2005 Oct 20;437(7062):1173-8.
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