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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Cytokeratin 8 Antibody   

Cytokeratin 8 Antibody

Purified Mouse Monoclonal Antibody

  • WB - Cytokeratin 8 Antibody AO1229a
    Figure 1: Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
  • IHC - Cytokeratin 8 Antibody AO1229a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb.
  • IHC - Cytokeratin 8 Antibody AO1229a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb
  • IF - Cytokeratin 8 Antibody AO1229a
    Figure 4: Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05787
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8A5D12
Isotype IgG1
Calculated MW 54kDa
Description Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Immunogen Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3856
Other Names Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8, KRT8, CYK8
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCytokeratin 8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT8
Synonyms CYK8
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Cellular Location Cytoplasm. Nucleus, nucleoplasm Nucleus matrix
Tissue Location Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Research Areas
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1. Cancer Genet Cytogenet. 2007 Oct 15;178(2):94-103. 2. Cancer Lett. 2008 Jul 8;265(2):188-96.

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$ 385.00
Cat# AO1229a
(40 western blots)
Availability: 7-10 days
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