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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   CD44 Antibody   

CD44 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CD44 Antibody AO1231a
    Figure 1: Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
  • WB - CD44 Antibody AO1231a
    Figure 2: Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.
  • IHC - CD44 Antibody AO1231a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.
  • IHC - CD44 Antibody AO1231a
    Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • IF - CD44 Antibody AO1231a
    Figure 5: Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IF - CD44 Antibody AO1231a
    Figure 6: Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • FC - CD44 Antibody AO1231a
    Figure 7: Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16070
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 8E2F3
Isotype IgG1
Calculated MW 82kDa
Description CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Immunogen Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 960
Other Names CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD44 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD44
Synonyms LHR, MDU2, MDU3, MIC4
Function Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with actin in membrane protrusions at wounding edges.
Tissue Location Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas Expression is repressed in neuroblastoma cells
Research Areas
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1. Exp Mol Pathol. 2007 Dec;83(3):341-6. 2. Mol Cell Proteomics. 2008 Mar;7(3):499-508.

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$ 300.00
Cat# AO1231a
(40 western blots)
Availability: 7-10 days
Bulk Size
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