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KLF15 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - KLF15 Antibody AO1260a
    Figure 1: Western blot analysis using KLF15 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
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  • ICC - KLF15 Antibody AO1260a
    Figure 2: Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using human IgGFc mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
    detail
  • IF - KLF15 Antibody AO1260a
    Figure 2: Confocal immunofluorescence analysis of HEK293 cells transfected with recombinant plasmid with human IgG Fc tag using mouse anti-human IgGFc antibody (green). Blue: DRAQ5 fluorescent DNA dye.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G8
Isotype IgG1
Calculated MW 44kDa
Description KLF15 (Kruppel-like factor 15), also known as KKLF, DKFZp779M1320. Entrez Protein NP_054798. Sequence analysis predicted that the 416 amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment.KLF15, a kidney-enriched Krueppel-like factor, is a transcriptonal activator that binds the CLCNKA promoter. KLF15, which is a nuclear protein, is expressedprimarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. KLF15 is an important regulator of GLUT4 in both adipose and muscle tissues.
Immunogen Purified recombinant fragment of human KLF15 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Dilution WB~~1/500 - 1/2000
ICC~~1:200~~1000
IF~~1:200~1000.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLF15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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References

1. Mol Cell Biol. 2000 Oct;20(19):7319-31. 2. Diabetologia. 2005 Jul;48(7):1315-22.

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$ 385.00
Cat# AO1260a
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