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ApoO Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - ApoO Antibody AO1262a
    Figure 1: Western blot analysis using ApoO mouse mAb against HepG2 (1) and 3T3L1(2) cell lysate.
  • IHC - ApoO Antibody AO1262a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Testis tissues using ApoO mouse mAb
  • ICC - ApoO Antibody AO1262a
    Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with FGFR4-hIgGFc using FGFR4 mouse mAb(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • IF - ApoO Antibody AO1262a
    Figure 2: Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with FGFR4-hIgGFc using anti-FGFR4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9BUR5
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 2F1
Isotype IgG1
Calculated MW 22.2kDa
Description ApoO: apolipoprotein O, also known as MYO25, FAM121B, MGC4825. Entrez Protein NP_077027. It is a chrondroitin-sulfate chain containing member of the apolipoprotein family and is an original glycoprotein up-regulated by diabetes in human Heart. Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.
Immunogen Purified recombinant fragment of ApoO expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 79135
Other Names Apolipoprotein O, Protein FAM121B, APOO, FAM121B
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
ICC~~1:200~~1000
IF~~1:200~1000.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsApoO Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOO
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein. Secreted. Mitochondrion. Golgi apparatus membrane. Endoplasmic reticulum membrane. Note=Exists in three distinct forms: a glycosylated and secreted form, an ER/Golgi-resident form and a non-glycosylated mitochondrial form
Tissue Location Expressed in all tissues examined. Up- regulated in diabetic heart.
Research Areas
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References

1. J Biol Chem. 2006 Nov 24;281(47):36289-302. 2. Genome Res. 2003 Oct;13(10):2265-70.

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$ 325.00
Cat# AO1262a
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