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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KI67 Antibody   

KI67 Antibody

Purified Mouse Monoclonal Antibody

  • IHC - KI67 Antibody AO1294a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 mouse mAb with DAB staining.
  • ICC - KI67 Antibody AO1294a
    Figure 1: Confocal immunofluorescence analysis of human peripheral blood lymphocyles (left) and mouse L1210 cells (right) using CD40 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • IF - KI67 Antibody AO1294a
    Figure 1: Confocal immunofluorescence analysis of human peripheral blood lymphocyles (left) and mouse L1210 cells (right) using anti-CD40 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46013
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4A1
Isotype IgG2b
Calculated MW 358kDa
Description Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
Immunogen Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4288
Other Names Antigen KI-67, MKI67
Dilution IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKI67 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MKI67
Function Thought to be required for maintaining cell proliferation.
Cellular Location Nucleus. Nucleus, nucleolus. Chromosome. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes
Research Areas
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1. Folia Histochem Cytobiol. 2007;45(4):357-66. 2. Tumori. 2008 May-Jun;94(3):389-97.

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$ 385.00
Cat# AO1294a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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