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TUBB3 Antibody

Purified Mouse Monoclonal Antibody

  • WB - TUBB3 Antibody AO1310a
    Figure 1: Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.
  • IF - TUBB3 Antibody AO1310a
    Figure 2: Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - TUBB3 Antibody AO1310a
    Figure 3: Flow cytometric analysis of A549 cells using TUBB3 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13509
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E9
Isotype IgG1
Calculated MW 50kDa
Description Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, α- and β-tubulin, are usually products of separate genes. The β-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III β-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III β-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
Immunogen Purified recombinant fragment of human TUBB3 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 10381
Other Names Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB3
Synonyms TUBB4
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
Research Areas
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1. Histopathology. 2007 Jun;50(7):949-52. 2. Neurochem Res. 2007 Aug;32(8):1387-98. 3. Exp Eye Res. 1995 Apr;60(4):385-400.

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$ 325.00
Cat# AO1310a
(40 western blots)
Availability: 7-10 days
Bulk Size
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