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NF-?B p65 AntibodyMouse Monoclonal Antibody to NF-?B p65
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AO1321a | 0.1ml 400 ul | 7-10 days | $ 325.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
NF-?B p65 Antibody - Product info | |
| Application | WB
|
| Primary Accession | Q04206 |
| Other Accession | NP_068810.3 |
| Reactivity | Human, Mouse |
| Isotype | Mouse IgG1 |
| Clone Names | 6H7 |
| Calculated MW | 60219 Da |
NF-?B p65 Antibody - Additional info | |
| Gene ID 5970 | |
| Other Names NFkappaB p65; p65; NFKB3; RELA | |
| Target/Specificity Purified recombinant fragment of human NF-?B p65 expressed in E. Coli. | |
| Dilution WB~~1:500 - 2000. | |
| Format Purified recombinant fragment of human NF-?B p65 expressed in E. Coli. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions NF-?B p65 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
NF-?B p65 Antibody - Protein Information | |
| Name RELA | |
| Synonyms NFKB3 | |
| Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1 | |
| Cellular Location Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity) Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation | |
NF-?B p65 Antibody - Related products
AP3176a: Phospho-NFKBp65-S536 Antibody
AP3178a: Phospho-NFKB-S536 Antibody
AP3717a: Phospho-NF kappa B-S276 Antibody
AP3749a: Phospho-NF kappa B-S536 Antibody
AP6826a: RELA Antibody (N-term)
AP6826c: RELA Antibody (Center)
LY10116a: RELA Over-expression Lysate
BP3176a: Phospho-NFKBp65-S536 Antibody Blocking Peptide
BP3178a: Phospho-NFKB-pS536 Antibody Blocking Peptide
BP3717a: Phospho-NF kappa B-S276 Antibody Blocking Peptide
BP6826a: RELA Antibody (N-term) Blocking Peptide
BP6826c: RELA Antibody (Center) Blocking Peptide
BP9125c: NF kappa B-S276 Antibody Blocking Peptide
BP9455a: NF kappa B-S276 Blocking Peptide
AT3616a: RELA Antibody (monoclonal) (M01)
AJ1537a: NF-kappaB Antibody (p65 subunit)
NF-?B p65 Antibody - Application data
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Figure 1: Western blot analysis using anti-NF-kB p65 mAb against Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
NF-?B p65 Antibody - Research Areas
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BACKGROUND
Transcription factors of the nuclear factor ? B (NF-?B)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-?B1 (p105/p50) and NF-?B2 (p100/p52). The most studied NF-?B complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-?B with its inhibitor, I?B. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I?B. This complex prevents nuclear translocation and activity of NF-?B. In response to stimuli such as cytokines, LPS, and viral infections, I?B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I?B/NF-?B complex, allowing the free heterodimeric NF-?B to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the ?B site within promoters and enhancers and functions as a transcriptional activator.
REFERENCES
1. Nature. 1997 Aug 7;388(6642):548-54.
2. Cell. 1998 Dec 11;95(6):749-58.
3. J Biol Chem. 2000 Jun 16;275(24):18180-7.
