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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   eNOS Antibody   

eNOS Antibody

Purified Mouse Monoclonal Antibody

  • IHC - eNOS Antibody AO1327a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.
  • IHC - eNOS Antibody AO1327a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.
  • WB - eNOS Antibody AO1327a
    Figure 1: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
  • FC - eNOS Antibody AO1327a
    Figure 4: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).
  • IF - eNOS Antibody AO1327a
    Figure 2:Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using anti-CTNNB1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29474
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6H2
Isotype IgG1
Calculated MW 133kDa
Description Endothelial nitric-oxide synthase (eNOS), also known as NOS3, it is an important enzyme in the cardiovascular system. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm.
Immunogen Purified recombinant fragment of human eNOS expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4846
Other Names Nitric oxide synthase, endothelial,, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3
Dilution IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionseNOS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOS3
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Cellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity
Tissue Location Platelets, placenta, liver and kidney.
Research Areas
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1. Nature. 1999 Jun 10;399(6736):601-5. 2. Oncol Rep. 2004 Nov;12(5):1007-11. 3. Breast Cancer Res Treat. 2008 May;109(1):181-2.

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$ 325.00
Cat# AO1327a
(40 western blots)
Availability: 7-10 days
Bulk Size
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