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AFP Antibody

Purified Mouse Monoclonal Antibody

  • WB - AFP Antibody AO1330a
    Figure 1: Western blot analysis using AFP mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02771
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6E6
Isotype IgG2b
Calculated MW 69kDa
Description AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Immunogen Purified recombinant fragment of human AFP expressed in E. Coli.
Formulation Antibody are purified by protein G affinity chromatography.
Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
Additional Information
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AFP
Synonyms HPAFP
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Cellular Location Secreted.
Tissue Location Plasma. Synthesized by the fetal liver and yolk sac
Research Areas
Citations (0)

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1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7160-4. 2. Pediatr Hematol Oncol. 2001 Dec;18(8):509-18. 3. Tumour Biol. 2002 Jul-Aug;23(4):202-11.

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$ 300.00
Cat# AO1330a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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