|Application ||WB, E|
|Description||AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.|
|Immunogen||Purified recombinant fragment of human AFP expressed in E. Coli.|
|Formulation||Antibody are purified by protein G affinity chromatography. |
Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
|Other Names||Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.|
|Tissue Location||Plasma. Synthesized by the fetal liver and yolk sac|
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Provided below are standard protocols that you may find useful for product applications.
1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7160-4. 2. Pediatr Hematol Oncol. 2001 Dec;18(8):509-18. 3. Tumour Biol. 2002 Jul-Aug;23(4):202-11.
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