|Application ||WB, IHC, FC, ICC, E|
|Description||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.|
|Immunogen||Purified recombinant fragment of human DDX4 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Probable ATP-dependent RNA helicase DDX4, 126.96.36.199, DEAD box protein 4, Vasa homolog, DDX4, VASA|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DDX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in germ cell development. May play a role in sperm motility.|
|Cellular Location||Cytoplasm. Cytoplasm, perinuclear region|
|Tissue Location||Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.|
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Provided below are standard protocols that you may find useful for product applications.
1. Proc Natl Acad Sci USA.2000 97(17):9585-90 2. Lab Invest.2002 82(2):159-66 3. Mol Reprod Dev.2004 67(1):1-7 4. Nat Genet.2004 36(1):40-5
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