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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   DDX4 Antibody   

DDX4 Antibody

Purified Mouse Monoclonal Antibody

  • WB - DDX4 Antibody AO1333a
    Figure 1: Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
  • IHC - DDX4 Antibody AO1333a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
  • IF - DDX4 Antibody AO1333a
    Figure 3: Immunofluorescence analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
  • FC - DDX4 Antibody AO1333a
    Figure 4: Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NQI0
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F9H5
Isotype IgG1
Calculated MW 76kDa
Description DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Immunogen Purified recombinant fragment of human DDX4 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 54514
Other Names Probable ATP-dependent RNA helicase DDX4,, DEAD box protein 4, Vasa homolog, DDX4, VASA
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX4
Synonyms VASA
Function May play a role in germ cell development. May play a role in sperm motility.
Cellular Location Cytoplasm. Cytoplasm, perinuclear region
Tissue Location Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Research Areas
Citations (0)

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1. Proc Natl Acad Sci USA.2000 97(17):9585-90 2. Lab Invest.2002 82(2):159-66 3. Mol Reprod Dev.2004 67(1):1-7 4. Nat Genet.2004 36(1):40-5

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$ 325.00
Cat# AO1333a
(40 western blots)
Availability: 7-10 days
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