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KRT15 Antibody

Purified Mouse Monoclonal Antibody

  • WB - KRT15 Antibody AO1335a
    Figure 1: Western blot analysis using KRT15 mouse mAb against A431 cell lysate.
  • IHC - KRT15 Antibody AO1335a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KRT15 mouse mAb
  • IF - KRT15 Antibody AO1335a
    Figure 3: Immunofluorescence analysis of HepG2(left) and PACN-1 (right) cells using KRT15 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • FC - KRT15 Antibody AO1335a
    Figure 4: Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19012
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6E7
Isotype IgG2a
Calculated MW 49kDa
Description The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
Immunogen Purified recombinant fragment of KRT15 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3866
Other Names Keratin, type I cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRT15, KRTB
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKRT15 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KRT15
Synonyms KRTB
Tissue Location Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate
Citations (0)

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1. J Invest Dermatol. 1999. 112(3):362-9 2. Exp Cell Res. 2006. 254(1):80-90 3. Mol Cell Biol. 2000. 24(8):3168-79

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$ 325.00
Cat# AO1335a
(40 western blots)
Availability: 7-10 days
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