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EGF Antibody

Purified Mouse Monoclonal Antibody

  • WB - EGF Antibody AO1339a
    Figure 1: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HEK293 cell lysate.
  • IHC - EGF Antibody AO1339a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung breast tissues using EGF mouse mAb
  • IHC - EGF Antibody AO1339a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01133
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 9D7F11
Isotype IgG1
Calculated MW 134kDa
Description Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 1950
Other Names Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF
Dilution WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EGF
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in kidney, salivary gland, cerebrum and prostate.
Research Areas
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1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405. 2. Oncogene. 2000 Mar 16;19(12):1509-18. 3. Nature. 2002 Mar 14;416(6877):183-7. 4. Radiat Res. 2003 Apr;159(4):439-52.

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$ 325.00
Cat# AO1339a
(40 western blots)
Availability: 7-10 days
Bulk Size
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