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BMP4 Antibody

Purified Mouse Monoclonal Antibody

  • WB - BMP4 Antibody AO1340a
    Figure 1: Western blot analysis using BMP4 mouse mAb against BMP4-hIgGFc transfected HEK293 cell lysate.
  • ICC - BMP4 Antibody AO1340a
    Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IF - BMP4 Antibody AO1340a
    Figure 2:Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12644
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 10F4B4
Isotype IgG1
Calculated MW 64kDa
Description The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors.BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
Immunogen Purified recombinant fragment of human BMP4 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 652
Other Names Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B, BMP4, BMP2B, DVR4
Dilution WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBMP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BMP4
Synonyms BMP2B, DVR4
Function Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines
Research Areas
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1. Genomics. 1995 Jun 10;27(3):559-60. 2. DNA Seq. 1995;5(5):273-5. 3. J Bone Miner Res. 2009 Dec;24(12):2039-49. 4. Stem Cells Dev. 2009 Nov;18(9):1283-92.

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$ 325.00
Cat# AO1340a
(40 western blots)
Availability: 7-10 days
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