|Application ||WB, FC, ICC, E|
|Description||Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).|
|Immunogen||Purified recombinant fragment of human NGFR expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NGFR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
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1. J Invest Dermatol. 1992 Dec;99(6):734-42. 2. Nature. 1997 Aug 7;388(6642):548-54. 3. Exp Neurol. 2002 Nov;178(1):104-11. 4. Science. 2004 May 7;304(5672):870-5.
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