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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CHD3 Antibody   

CHD3 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CHD3 Antibody AO1344a
    Figure 1: Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
  • IHC - CHD3 Antibody AO1344a
    Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
  • IF - CHD3 Antibody AO1344a
    Figure 3: Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - CHD3 Antibody AO1344a
    Figure 4: Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12873
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 2G4
Isotype IgG1
Calculated MW 226kDa
Description This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Immunogen Purified recombinant fragment of human CHD3 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.
Additional Information
Gene ID 1107
Other Names Chromodomain-helicase-DNA-binding protein 3, CHD-3,, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHD3
Function Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis
Tissue Location Widely expressed.
Research Areas
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1. Virus Res. 2003 Dec;98(1):83-91. 2. Mol Cell. 2004 Sep 24;15(6):853-65. 3. J Biol Chem. 2008 Dec 12;283(50):34976-82.

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$ 325.00
Cat# AO1344a
(40 western blots)
Availability: 7-10 days
Bulk Size
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