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CHD3 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | Q12873 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2G4 |
| Isotype | IgG1 |
| Calculated MW | 226kDa |
| Description | This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. |
| Immunogen | Purified recombinant fragment of human CHD3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
| Gene ID | 1107 |
|---|---|
| Other Names | Chromodomain-helicase-DNA-binding protein 3, CHD-3, 3.6.4.12, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3 |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 IF~~1/200 - 1/1000 FC~~1/200 - 1/400 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CHD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHD3 |
|---|---|
| Function | ATP-dependent helicase that binds and distorts nucleosomal DNA (PubMed:28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:9804427, PubMed:30397230, PubMed:16428440, PubMed:28977666). Involved in transcriptional repression as part of the NuRD complex (PubMed:27068747). Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity (PubMed:17626165). |
| Cellular Location | Nucleus, PML body. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Associates with centrosomes in interphase and mitosis (PubMed:17626165). Localizes to sites of DNA damage (PubMed:28977666) |
| Tissue Location | Widely expressed. |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Virus Res. 2003 Dec;98(1):83-91. 2. Mol Cell. 2004 Sep 24;15(6):853-65. 3. J Biol Chem. 2008 Dec 12;283(50):34976-82.
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$ 325.00
Cat# AO1344a
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