|Application ||WB, IHC, FC, ICC, E|
|Description||This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.|
|Immunogen||Purified recombinant fragment of human CHD3 expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide.|
|Other Names||Chromodomain-helicase-DNA-binding protein 3, CHD-3, 18.104.22.168, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Mi2-alpha, Zinc finger helicase, hZFH, CHD3|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis|
|Tissue Location||Widely expressed.|
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1. Virus Res. 2003 Dec;98(1):83-91. 2. Mol Cell. 2004 Sep 24;15(6):853-65. 3. J Biol Chem. 2008 Dec 12;283(50):34976-82.
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