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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Rab25 Antibody   

Rab25 Antibody

Purified Mouse Monoclonal Antibody

  • WB - Rab25 Antibody AO1350a
    Figure 1: Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
  • IHC - Rab25 Antibody AO1350a
    Figure 2: Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
  • FC - Rab25 Antibody AO1350a
    Figure 3: Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).
  • IF - Rab25 Antibody AO1350a
    Figure 4: Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P57735
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 3F12
Isotype IgG1
Calculated MW 24kDa
Description Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
Immunogen Purified recombinant fragment of Rab25 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 57111
Other Names Ras-related protein Rab-25, CATX-8, RAB25, CATX8
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRab25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB25 (HGNC:18238)
Synonyms CATX8
Function Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Note=Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips
Tissue Location Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue
Research Areas
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1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422 2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146

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$ 325.00
Cat# AO1350a
(40 western blots)
Availability: 7-10 days
Bulk Size
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