|Application ||WB, IHC, FC, ICC, E|
|Description||Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.|
|Immunogen||Purified recombinant fragment of Rab25 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Ras-related protein Rab-25, CATX-8, RAB25, CATX8|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Rab25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, pseudopodium membrane. Cytoplasmic vesicle. Note=Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips|
|Tissue Location||Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue|
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1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422 2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146
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