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ATP2C1 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P98194 |
| Reactivity | Human, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 4G12 |
| Isotype | IgG1 |
| Calculated MW | 100kDa |
| Description | ATP2C1, also known as PMR1, it belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. The human homologue, ATP2C1 (alsodesignated SPLA in rat), also regulates the transport of calcium in the Golgicomplex and is related to other P-type ATPases family members, such as thesarco(endo)plasmic calcium ATPase (SERCA) and the plasma membrane calciumATPase (PCMA). ATP2C1 is a transmembrane protein that exists as twosplice variants, which vary by 20 amino acids. Defects in ATP2C1 cause Hailey-Hailey disease, which is an autosomal dominant disorder that is characterized by blisters and erosions of the skin. These findings provide further evidence that PMR1 plays a key role in maintaining the integrity of the epidermis by controlling intracellular calcium signaling. |
| Immunogen | Purified recombinant fragment of ATP2C1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 27032 |
|---|---|
| Other Names | Calcium-transporting ATPase type 2C member 1, ATPase 2C1, 3.6.3.8, ATP-dependent Ca(2+) pump PMR1, ATP2C1, KIAA1347, PMR1L |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/500 - 1/2000 FC~~1:200~~400 IF~~1:200~1000. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ATP2C1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP2C1 {ECO:0000303|PubMed:10615129, ECO:0000312|HGNC:HGNC:13211} |
|---|---|
| Function | ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway (PubMed:16192278, PubMed:30923126, PubMed:21187401, PubMed:12707275, PubMed:20439740). Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:16192278, PubMed:16332677, PubMed:30923126). Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology (PubMed:20439740, PubMed:14632183). Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity (PubMed:14632183, PubMed:10615129, PubMed:20439740). Participates in Ca(2+) and Mn(2+) ions uptake into the Golgi store of hippocampal neurons and regulates protein trafficking required for neural polarity (By similarity). May also play a role in the maintenance of Ca(2+) and Mn(2+) homeostasis and signaling in the cytosol while preventing cytotoxicity (PubMed:21187401). |
| Cellular Location | Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Note=During neuron differentiation, shifts from juxtanuclear Golgi position to multiple Golgi structures distributed over the neural soma with a predominance in the apical dendritic trunk {ECO:0000250|UniProtKB:Q80XR2} |
| Tissue Location | Found in most tissues except colon, thymus, spleen and leukocytes (PubMed:15831496). Expressed in keratinocytes (at protein level) (PubMed:15831496, PubMed:14632183) |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. J Invest Dermatol. 2005 Nov;125(5):933-5. 2. J Dermatol Sci. 2006 Aug;43(2):150-1. 3. Dermatology. 2007;215(4):277-83.
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$ 325.00
Cat# AO1354a
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