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GOT2 Antibody

Purified Mouse Monoclonal Antibody

  • WB - GOT2 Antibody AO1357a
    Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
  • IF - GOT2 Antibody AO1357a
    Figure 2: Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • IHC - GOT2 Antibody AO1357a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C mouse mAb
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00505
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3E9
Isotype IgG1
Calculated MW 47kDa
Description Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic andinner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and theurea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Immunogen Purified recombinant fragment of human GOT2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2806
Other Names Aspartate aminotransferase, mitochondrial, mAspAT,,, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, GOT2
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGOT2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GOT2
Function Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Cellular Location Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane
Research Areas
Citations (0)

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1. Hepatology. 1998 Apr;27(4):1064-74. 2. Cell. 2005 Sep 23;122(6):957-68. 3. Psychiatr Genet. 2007 Oct;17(5):314.

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$ 325.00
Cat# AO1357a
(40 western blots)
Availability: 7-10 days
Bulk Size
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