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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Pirh2 Antibody   

Pirh2 Antibody

Purified Mouse Monoclonal Antibody

  • WB - Pirh2 Antibody AO1358a
    Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
  • IHC - Pirh2 Antibody AO1358a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
  • FC - Pirh2 Antibody AO1358a
    Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
  • IF - Pirh2 Antibody AO1358a
    Figure 4: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96PM5
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 1H10
Isotype IgG1
Calculated MW 30kDa; 60kDa (homodimer)
Description Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Immunogen Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 25898
Other Names RING finger and CHY zinc finger domain-containing protein 1, 6.3.2.-, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPirh2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RCHY1
Synonyms ARNIP, CHIMP, PIRH2, RNF199, ZNF363
Function Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
Cellular Location Nucleus. Nucleus speckle. Cytoplasm.
Research Areas
Citations (0)

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1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50. 2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21. 3. Exp Cell Res. 2006 Oct 15;312(17):3370-8. 4. Cell. 2003 Mar 21;112(6):779-91.

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$ 325.00
Cat# AO1358a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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