|Application ||WB, IHC, FC, ICC, E|
|Calculated MW||30kDa; 60kDa (homodimer)|
|Description||Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.|
|Immunogen||Purified recombinant fragment of human Pirh2 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||RING finger and CHY zinc finger domain-containing protein 1, 6.3.2.-, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Pirh2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ARNIP, CHIMP, PIRH2, RNF199, ZNF363|
|Function||Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.|
|Cellular Location||Nucleus. Nucleus speckle. Cytoplasm.|
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1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50. 2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21. 3. Exp Cell Res. 2006 Oct 15;312(17):3370-8. 4. Cell. 2003 Mar 21;112(6):779-91.
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