|Application ||WB, ICC, E|
|Description||Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.|
|Immunogen||Purified recombinant fragment of human EGF expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||Expressed in kidney, salivary gland, cerebrum and prostate.|
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1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405. 2. Oncogene. 2000 Mar 16;19(12):1509-18. 3. Nature. 2002 Mar 14;416(6877):183-7. 4. Radiat Res. 2003 Apr;159(4):439-52.
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