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CD247 Antibody

Purified Mouse Monoclonal Antibody

  • IF - CD247 Antibody AO1370a
    Figure3: Immunofluorescence analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • WB - CD247 Antibody AO1370a
    Figure 1: Western blot analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.
  • IHC - CD247 Antibody AO1370a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-CD247 mouse mAb
  • FC - CD247 Antibody AO1370a
    Figure 4: Flow cytometric analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20963
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4B10
Isotype IgG1
Calculated MW 18kDa
Description The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human CD247 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 919
Other Names T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ
Dilution IF~~1/200 - 1/1000
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD247 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD247
Synonyms CD3Z, T3Z, TCRZ
Function Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Cellular Location Membrane; Single-pass type I membrane protein
Citations (0)

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1. J Immunol. 2002 Aug 15;169(4):1705-12. 2. Arthritis Rheum. 2003 Jul;48(7):1948-55. 3. Nat Methods. 2005 Aug;2(8):591-8.

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$ 325.00
Cat# AO1370a
(40 western blots)
Availability: 7-10 days
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