|Application ||WB, IHC, E|
|Description||The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.|
|Immunogen||Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, 126.96.36.199, Endoribonuclease, 3.1.26.-, ERN1 (HGNC:3449)|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ERN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto- activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein|
|Tissue Location||Ubiquitously expressed. High levels observed in pancreatic tissue.|
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1. Biochem Biophys Res Commun. 2004 Apr 30;317(2):390-6. 2. Mol Cell Biol. 2005 Sep;25(17):7522-33. 3. Science. 2007 Nov 9;318(5852):944-9.
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