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GATA1 Antibody

Mouse Monoclonal Antibody to GATA1

     
  • WB - GATA1 Antibody AO1381b
    Figure 1: Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate.
  • IHC - GATA1 Antibody AO1381b
    Figure 3: Immunohistochemical analysis of paraffin-embedded pancreatic cancer, using GATA1 mouse mAb with DAB staining.
  • IF - GATA1 Antibody AO1381b
    Figure 2:Immunofluorescence analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P15976
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4F5
Isotype Mouse IgG2b
Calculated MW 42751 Da
Description erythroid specific transcription factor,GATA sequence binding protein (same as EryF1,NF-E1,EF-1,EF gamma-a,GF1),expressed in RBC,mast cell,megakaryocyte,hematopoietic progenitor cell,testis,associated with a special class of nuclear bodies,activated by erythropoietin,inactivated by coactivation of DEATH receptors (TNFRSF6) mediated caspase cleavage Tissue specificity: Erythrocytes
Immunogen Purified recombinant fragment of human GATA1 expressed in E. Coli.
Additional Information
Gene ID 2623
Other Names Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein, GATA1, ERYF1, GF1
Target/Specificity Purified recombinant fragment of human GATA1 expressed in E. Coli.
Dilution WB~~1:500~~2000
IHC~~1:200~~1000
IF~~1:200~1000.
Format Ascitic fluid containing 0.03% sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGATA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GATA1
Synonyms ERYF1, GF1
Function Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Cellular Location Nucleus.
Tissue Location Erythrocytes..
Research Areas
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References

1. Cancer Res. 2009 Apr 15;69(8):3681-8.

2. J Bone Miner Res. 2009 Dec;24(12):2039-49.

3. Blood. 2010 Jun 3;115(22):4367-76.

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$ 325.00
Cat# AO1381b
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