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ALPP Antibody

Purified Mouse Monoclonal Antibody

  • WB - ALPP Antibody AO1414a
    Figure 1: Western blot analysis using ALPP mouse mAb against HepG2 (1), A431 (2) and MCF-7 (3) cell lysate.
  • IHC - ALPP Antibody AO1414a
    Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (A), stomach cancer (B) using ALPP mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05187
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E5
Isotype IgG2b
Calculated MW 58kDa
Description There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Immunogen Purified recombinant fragment of human ALPP expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 250
Other Names Alkaline phosphatase, placental type,, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, ALPP, PLAP
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALPP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PLAP
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location Detected in placenta (at protein level).
Research Areas
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1. J Cell Biochem. 2003 Apr 1;88(5):1025-37. 2. J Mol Biol. 2005 Jul 15;350(3):441-51.

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$ 325.00
Cat# AO1414a
(40 western blots)
Availability: 7-10 days
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