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CDC27 Antibody

Purified Mouse Monoclonal Antibody

  • WB - CDC27 Antibody AO1417a
    Figure 1: Western blot analysis using CDC27 mouse mAb against CDC27(AA: 724-830)-hIgGFc transfected HEK293 cell.
  • IHC - CDC27 Antibody AO1417a
    Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and colon cancer tissues (right) using CDC27 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30260
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C12
Isotype IgG1
Calculated MW 91kDa
Description Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Immunogen Purified recombinant fragment of human CDC27 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 996
Other Names Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, H-NUC, CDC27, ANAPC3, D0S1430E, D17S978E
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDC27 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC27
Synonyms ANAPC3, D0S1430E, D17S978E
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Cellular Location Nucleus.
Research Areas
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1. Mol Cell Biol. 2004 May;24(9):3577-87. 2. J Biol Chem. 2004 Oct 1;279(40):42128-38.

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$ 325.00
Cat# AO1417a
(40 western blots)
Availability: 7-10 days
Bulk Size
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