|Application ||WB, FC, E|
|Description||Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells .PAX6 is the most researched of the PAX genes and appears throughout the literature as a"master control"gene for the development of eyes and other sensory organs, certain neural and epidermal tissues as well as other homologous structures, usually derived from ectodermal tissues. This transcription factor is most famous for its use in the interspecifically induced expression of ectopic eyes and is of medical importance because heterozygous mutants produce a wide spectrum of ocular defects such as Aniridia in humans. This gene encodes paired box gene 6, one of many human homologues of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause aniridia as well as Peter's anomaly, both ocular diseases.|
|Immunogen||Purified recombinant fragment of human PAX6 expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2|
|Dilution||WB~~1/500 - 1/2000|
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAX6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.|
|Tissue Location||Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form|
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Provided below are standard protocols that you may find useful for product applications.
1.Invest Ophthalmol Vis Sci. 2009 Jun;50(6):2581-90. 2.J Biol Chem. 2009 Oct 2;284(40):27524-32. 3.J Biol Chem. 2010 Jan 22;285(4):2527-36.
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