|Application ||WB, IHC, FC, ICC, E|
|Description||KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate- specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase.|
|Immunogen||Purified recombinant fragment of human KLHL13 expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Kelch-like protein 13, BTB and kelch domain-containing protein 2, KLHL13, BKLHD2, KIAA1309|
|Dilution||WB~~1/500 - 1/2000|
IF~~1/200 - 1/1000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLHL13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.|
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1. DNA Res. 2000 Feb 28;7(1):65-73. 2. Genome Res. 2004 Sep;14(9):1711-8. 3. Cell. 2009 Jul 23;138(2):389-403.
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