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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BCL-2 Antibody   

BCL-2 Antibody

Purified Mouse Monoclonal Antibody

  • FC - BCL-2 Antibody AO1430a
    Figure 1: Flow cytometric analysis of 3T3L1 cells using BCL-2 mouse mAb (green) and negative control (purple).
  • IF - BCL-2 Antibody AO1430a
    Figure 2: Immunofluorescence analysis of NIH/3T3 cells using BCL-2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • IHC - BCL-2 Antibody AO1430a
    Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues (left) and human brain tissues (right) using BCL-2 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10415
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 8E12
Isotype IgG1
Calculated MW 26kDa
Description This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Tissue specificity: Expressed in a variety of tissues.
Immunogen Synthetic peptide corresponding to residues surrounding BCL-2, conjugated to KLH.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 596
Other Names Apoptosis regulator Bcl-2, BCL2
Dilution FC~~1/200 - 1/400
IF~~1/200 - 1/1000
IHC~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBCL-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BCL2
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Cellular Location Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location Expressed in a variety of tissues.
Research Areas
Citations (0)

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1. J Biol Chem. 2010 Mar 26;285(13):9770-9. 2. Pharmacogenomics J. 2010 Feb 16. 3. J Int Med Res. 2009 Nov-Dec;37(6):1868-76.

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$ 325.00
Cat# AO1430a
(40 western blots)
Availability: 7-10 days
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