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KLF4 Antibody

Purified Mouse Monoclonal Antibody

  • WB - KLF4 Antibody AO1434a
    Figure 1: Western blot analysis using KLF4 mouse mAb against KLF4 (aa2-470)-hIgGFc transfected HEK293 (1) and HEK293 (2) cell lysate.
  • IHC - KLF4 Antibody AO1434a
    Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and human rectum tissues (right) using KLF4 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43474
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1E5
Isotype IgG1
Calculated MW 55kDa
Description Kruppel-like factor 4 (gut),endothelial Kruppel-like zinc finger protein. May act as a transcriptional activator. Binds the CACCC core sequence. May be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract.
Immunogen Purified recombinant fragment of human KLF4 (AA: 2-470) expressed in E. Coli.

Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 9314
Other Names Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLF4
Synonyms EZF, GKLF
Function Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Cellular Location Nucleus.
Citations (0)

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1. Mol Hum Reprod. 2009 Aug;15(8):479-88. 2. Circ Res. 2009 Sep 25;105(7):648-56.

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$ 325.00
Cat# AO1434a
(40 western blots)
Availability: 7-10 days
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