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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RICTOR Antibody   

RICTOR Antibody

Purified Mouse Monoclonal Antibody

  • WB - RICTOR Antibody AO1443a
    Figure 1: Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysate.
  • IF - RICTOR Antibody AO1443a
    Figure 2: Immunofluorescence analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • IHC - RICTOR Antibody AO1443a
    Figure 3: Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.
  • FC - RICTOR Antibody AO1443a
    Figure 4: Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6R327
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 7B3
Isotype IgG1
Calculated MW 192kDa
Description Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals . mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1 and rictor . The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro . This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes . This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR .
Immunogen Purified recombinant fragment of human RICTOR expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 253260
Other Names Rapamycin-insensitive companion of mTOR, AVO3 homolog, hAVO3, RICTOR {ECO:0000312|EMBL:EAW55980.1}
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRICTOR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RICTOR {ECO:0000312|EMBL:EAW55980.1}
Function Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Research Areas
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1. Genes Dev. 2006 Oct 15;20(20):2820-32. 2. Biochem Biophys Res Commun. 2008 Aug 8;372(4):578-83.

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$ 325.00
Cat# AO1443a
(40 western blots)
Availability: 7-10 days
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