|Application ||WB, E|
|Description||The modification of proteins with ubiquitin is an important cellular mechanism for targeting.Tissue specificity: Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein.|
|Immunogen||Purified recombinant fragment of human UBE1L expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog, UBA7, UBE1L, UBE2|
|Dilution||WB~~1/500 - 1/2000|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE1L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates ubiquitin by first adenylating with ATP its C- terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin- E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.|
|Tissue Location||Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines|
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Provided below are standard protocols that you may find useful for product applications.
1. Cancer Res. 1992 Mar 15;52(6):1536-41 2. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6071-5. 3. Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3806-11.
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