|Application ||WB, IHC, E|
|Description||Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.|
|Immunogen||Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Antigen KI-67, MKI67|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KI67 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thought to be required for maintaining cell proliferation.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Chromosome. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes|
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Provided below are standard protocols that you may find useful for product applications.
1. Folia Histochem Cytobiol. 2007;45(4):357-66. 2. Tumori. 2008 May-Jun;94(3):389-97.
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