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CD276 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - CD276 Antibody AO1455a
    Figure 1: Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - CD276 Antibody AO1455a
    Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.
  • FC - CD276 Antibody AO1455a
    Figure 3: Flow cytometric analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession Q5ZPR3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A1
Isotype IgG1
Calculated MW 57kDa
Description Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558). May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Immunogen Purified recombinant fragment of human CD276 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 80381
Other Names CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276, CD276, B7H3
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD276 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD276
Synonyms B7H3
Function May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta
Research Areas
Citations (0)

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References

1. Genome Res. 2004 Oct;14(10B):2121-7. 2. Cell Mol Immunol. 2005 Aug;2(4):307-11.

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$ 325.00
Cat# AO1455a
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