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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GFAP Antibody   

GFAP Antibody

Purified Mouse Monoclonal Antibody

  • WB - GFAP Antibody AO1460a
    Figure 1: Western blot analysis using GFAP mouse mAb against A431 (1), SK-N-SH (2) and PC12 (3) cell lysate.
  • IHC - GFAP Antibody AO1460a
    Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues using GFAP mouse mAb with DAB staining
  • IF - GFAP Antibody AO1460a
    Figure 3: Immunofluorescence analysis of paraffin-embedded lobe of brain tissues using GFAP mouse mAb (green).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14136
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A6
Isotype IgG1
Calculated MW 50kDa
Description GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.Tissue specificity: Expressed in cells lacking fibronectin.ABCAM:It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves.In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Immunogen Purified recombinant fragment of human GFAP expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Citations (0)

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1. Acta Neuropathol. 2009 Jun;117(6):667-75. 2. Schizophr Res. 2009 Jul;112(1-3):54-64.

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$ 325.00
Cat# AO1460a
(40 western blots)
Availability: 7-10 days
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