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KLHL22 Antibody

Purified Mouse Monoclonal Antibody

  • WB - KLHL22 Antibody AO1461a
    Figure 1: Western blot analysis using KLHL22 mouse mAb against mouse brain tissues lysate.
  • IF - KLHL22 Antibody AO1461a
    Figure 2: Immunofluorescence analysis of U251 cells using KLHL22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q53GT1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7B10
Isotype IgG1
Calculated MW 72kDa
Description KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophilakelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL22 protein contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL22 that are produced as a result of alternative splicing events.
Immunogen Purified recombinant fragment of human KLHL22 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 84861
Other Names Kelch-like protein 22, KLHL22
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLHL22 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLHL22
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Mainly. cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase
Research Areas
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1. Gene. 1994 Jan 28;138(1-2):171-4. 2. Nat Genet. 2004 Jan;36(1):40-5. 3. Cell. 2009 Jul 23;138(2):389-403.

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$ 325.00
Cat# AO1461a
(40 western blots)
Availability: 7-10 days
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