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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   STAT3 Antibody   

STAT3 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - STAT3 Antibody AO1474a
    Figure 1: Western blot analysis using STAT3 mouse mAb against Hela (1),NIH/3T3 (2), Jurkat (3), PC-12 (4) and COS7 (5) cell lysate.
  • IHC - STAT3 Antibody AO1474a
    Figure 2: Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.
  • IF - STAT3 Antibody AO1474a
    Figure 3: Immunofluorescence analysis of Hela cells using STAT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P40763
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3B5
Isotype IgG1
Calculated MW 88kDa
Description The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development . Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage. It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Immunogen Purified recombinant fragment of human STAT3 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 6774
Other Names Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTAT3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STAT3 (HGNC:11364)
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3 Identified in a complex with LYN and PAG1
Tissue Location Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
Citations (0)

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References

1. J Mol Graph Model. 2009 Nov;28(4):347-56. 2. Bone. 2010 Feb;46(2):524-33.

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$ 325.00
Cat# AO1474a
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