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KDM3A Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - KDM3A Antibody AO1486a
    Figure 1: Western blot analysis using KDM3A mouse mAb against Hela (1) and HepG2 (2) cell lysate.
    detail
  • IF - KDM3A Antibody AO1486a
    Figure 2: Immunofluorescence analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q9Y4C1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E1
Isotype IgG1
Calculated MW 147kDa
Description This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and alpha-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.
Immunogen Purified recombinant fragment of human KDM3A expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 55818
Other Names Lysine-specific demethylase 3A, 1.14.11.-, JmjC domain-containing histone demethylation protein 2A, Jumonji domain-containing protein 1A, KDM3A, JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA
Dilution WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKDM3A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KDM3A
Synonyms JHDM2A, JMJD1, JMJD1A, KIAA0742, TSGA
Function Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Cellular Location Cytoplasm. Nucleus. Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).
Research Areas
Citations (0)
citation

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References

1. DNA Res. 1998 Oct 30;5(5):277-86. 2. Proc Natl Acad Sci U S A.2004 Aug 17;101(33):12130-5. 3. Nature. 2005 Apr 7;434(7034):724-31.

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$ 325.00
Cat# AO1486a
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