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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   FOXD3 Antibody   

FOXD3 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - FOXD3 Antibody AO1493a
    Figure 1: Western blot analysis using FOXD3 mouse mAb against NTERA-2 (1), HUVE-12 (2), HEK293 (3), Hela (4), Jurkat (5), K562 (6), RAW264.7 (7), NIH/3T3 (8), and COS7 (9) cell lysate.
  • IHC - FOXD3 Antibody AO1493a
    Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and ovarian cancer tissues (right) using FOXD3 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9UJU5
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 5G9
Isotype IgG1
Calculated MW 50kDa
Description FoxD3 is a member of the Forkhead Box family and is characterized by a winged-helix DNA-binding structure and the important role it plays in embryonic development . This transcriptional regulator is required for the maintenance of pluripotency in the pre-implantation and peri-implantation stages of mouse embryonic development and is also required for trophoblast formation. FoxD3 is required for the maintenance of the mammalian neural crest; FoxD3(-/-) mouse embryos fail around the time of implantation with loss of neural crest-derived structures. FoxD3 also forms a regulatory network with Oct-4 and NANOG to maintain the pluripotency of ES cells. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. Tissue specificity: Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined.
Immunogen Purified recombinant fragment of human FOXD3 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 27022
Other Names Forkhead box protein D3, HNF3/FH transcription factor genesis, FOXD3, HFH2
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFOXD3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOXD3
Synonyms HFH2
Function Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. Also acts as a transcriptional activator. Promotes development of neural crest cells from neural tube progenitors. Restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. Required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis.
Cellular Location Nucleus.
Tissue Location Expressed in chronic myeloid leukemia, Jurkat T-cell leukemia and teratocarcinoma cell lines, but not in any other cell lines or normal tissues examined
Research Areas
Citations (0)

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References

1. J Neuroimmune Pharmacol. 2009 Mar;4(1):103-15. 2. Nature. 2006 May 18;441(7091):315-21.

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$ 325.00
Cat# AO1493a
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