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CA9 Antibody

Purified Mouse Monoclonal Antibody

     
  • WB - CA9 Antibody AO1500a
    Figure 1: Western blot analysis using CA9 mouse mAb against Hela (1) and A549 (2) cell lysate.
  • IHC - CA9 Antibody AO1500a
    Figure 2: Immunohistochemical analysis of paraffin-embedded stomach tissues (left) and colon tissues (right) using CA9 mouse mAb with DAB staining.
  • FC - CA9 Antibody AO1500a
    Figure 3: Flow cytometric analysis of NTERA-2 cells using CA9 mouse mAb (green) and negative control (purple).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession Q16790
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2D3
Isotype IgG1
Calculated MW 50kDa
Description CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Immunogen Purified recombinant fragment of human CA9 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 768
Other Names Carbonic anhydrase 9, 4.2.1.1, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, CAIX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9, G250, MN
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCA9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CA9
Synonyms G250, MN
Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Cellular Location Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus
Tissue Location Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa
Research Areas
Citations (0)

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References

1. Br J Cancer. 2008 Sep 2;99(5):727-33. 2. Pathol Res Pract. 2009;205(1):1-9.

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$ 325.00
Cat# AO1500a
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