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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SMAD5 Antibody   

SMAD5 Antibody

Purified Mouse Monoclonal Antibody

  • WB - SMAD5 Antibody AO1502a
    Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
  • IHC - SMAD5 Antibody AO1502a
    Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
  • IF - SMAD5 Antibody AO1502a
    Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - SMAD5 Antibody AO1502a
    Figure 4: Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99717
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 3H9
Isotype IgG1
Calculated MW 52kDa
Description Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Immunogen Purified recombinant fragment of human SMAD5 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4090
Other Names Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMAD5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD5
Synonyms MADH5
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4
Tissue Location Ubiquitous.
Research Areas
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1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32. 2. Nat Cell Biol. 2008 May;10(5):567-74.

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$ 325.00
Cat# AO1502a
(40 western blots)
Availability: 7-10 days
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