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SMAD5 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | Q99717 |
| Reactivity | Human, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 3H9 |
| Isotype | IgG1 |
| Calculated MW | 52kDa |
| Description | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous. |
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Gene ID | 4090 |
|---|---|
| Other Names | Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5 |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 IF~~1/200 - 1/1000 FC~~1/200 - 1/400 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SMAD5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMAD5 |
|---|---|
| Synonyms | MADH5 |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis (PubMed:16516194, PubMed:12064918). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed:9442019). In turn, the hetero-trimeric complex recognizes cis- regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed:33510867). Non- phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes (PubMed:28675158). Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis (PubMed:28675158). |
| Cellular Location | Cytoplasm. Nucleus Mitochondrion. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 |
| Tissue Location | Ubiquitous. |
Research Areas
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
References
1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32. 2. Nat Cell Biol. 2008 May;10(5):567-74.
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$ 325.00
Cat# AO1502a
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