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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   MAP2K4 Antibody   

MAP2K4 Antibody

Purified Mouse Monoclonal Antibody

  • E - MAP2K4 Antibody AO1520a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - MAP2K4 Antibody AO1520a
    Figure 1: Western blot analysis using MAP2K4 mouse mAb against HepG2 (1), K562 (2), and HEK293 (3) cell lysate.
  • IHC - MAP2K4 Antibody AO1520a
    Figure 2: Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K4 mouse mAb with DAB staining.
  • FC - MAP2K4 Antibody AO1520a
    Figure 3: Flow cytometric analysis of K562 cells using MAP2K4 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P45985
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5H4
Isotype IgG1
Calculated MW 42kDa
Description This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.Tissue specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues .
Immunogen Purified recombinant fragment of human MAP2K4 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 6416
Other Names Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4,, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK, MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2K4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K4
Synonyms JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1,
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
Cellular Location Cytoplasm. Nucleus
Tissue Location Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues
Research Areas
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1. J Biol Chem. 2009 Jan 2;284(1):685-95. 2. J Immunol. 2008 Sep 1;181(5):3252-8.

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$ 385.00
Cat# AO1520a
(40 western blots)
Availability: 7-10 days
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