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MSI1 Antibody

Purified Mouse Monoclonal Antibody

  • E - MSI1 Antibody AO1529a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - MSI1 Antibody AO1529a
    Figure 1: Western blot analysis using MSI1 mAb against human MSI1 (AA: 1-203) recombinant protein. (Expected MW is 48.5 kDa)
  • WB - MSI1 Antibody AO1529a
    Figure 2: Western blot analysis using MSI1 mouse mAb against NTERA-2 cell lysate.
  • FC - MSI1 Antibody AO1529a
    Figure 3: Flow cytometric analysis of PC-2 cells using MSI1 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43347
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A12
Isotype IgG1
Calculated MW 39kDa
Description This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas.Musashi-1 is frequently used as a marker for proliferating neural precursor cells, it is also expressed in epithelial stem cells including intestinal and mammary gland stem cells.Tissue specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Immunogen Purified recombinant fragment of human MSI1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4440
Other Names RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMSI1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSI1
Function RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Cellular Location Cytoplasm. Nucleus
Tissue Location Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
Research Areas
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1. BMC Cancer. 2008 Apr 18;8:108. 2. Gastroenterology. 2008 May;134(5):1448-58.

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$ 385.00
Cat# AO1529a
(40 western blots)
Availability: 7-10 days
Bulk Size
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