|Application ||WB, IHC, E|
|Description||E7 transforming protein (Human papillomavirus type 16).|
|Immunogen||Purified recombinant fragment of E7 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Protein E7, E7|
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||E7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription (By similarity).|
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1. Methods Mol Med. 2005;119:363-79. 2. Viral Immunol. 2006 Summer;19(3):468-80.
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