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GRK2 Antibody

Purified Mouse Monoclonal Antibody

  • E - GRK2 Antibody AO1550a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - GRK2 Antibody AO1550a
    Figure 1: Western blot analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
  • IHC - GRK2 Antibody AO1550a
    Figure 2: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.
  • IF - GRK2 Antibody AO1550a
    Figure 3: Immunofluorescence analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25098
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3F8
Isotype IgG1
Calculated MW 80kDa
Description The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. (provided by RefSeq)Tissue specificity: Expressed in peripheral blood leukocytes
Immunogen Purified recombinant fragment of human GRK2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 156
Other Names Beta-adrenergic receptor kinase 1, Beta-ARK-1,, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGRK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BARK, BARK1, GRK2
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P21146}. Cell membrane {ECO:0000250|UniProtKB:P21146}
Tissue Location Expressed in peripheral blood leukocytes.
Research Areas
Citations (0)

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1. Mol Biol Cell. 2008 Jul;19(7):2973-83. 2. Biochemistry. 2009 May 26;48(20):4285-93.

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$ 385.00
Cat# AO1550a
(40 western blots)
Availability: 7-10 days
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