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OTX2 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - OTX2 Antibody AO1568a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - OTX2 Antibody AO1568a
    Figure 1: Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.
  • WB - OTX2 Antibody AO1568a
    Figure 2: Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)
  • IHC - OTX2 Antibody AO1568a
    Figure 3: Immunohistochemical analysis of paraffin-embedded colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.
  • IHC - OTX2 Antibody AO1568a
    Figure 4: Immunohistochemical analysis of paraffin-embedded stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.
  • IF - OTX2 Antibody AO1568a
    Figure 5: Immunofluorescence analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, E
Primary Accession P32243
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H12G8B2
Isotype IgG1
Calculated MW 32kDa
Description This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development.Tissue specificity: Expressed in brain.
Immunogen Purified recombinant fragment of human OTX2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 5015
Other Names Homeobox protein OTX2, Orthodenticle homolog 2, OTX2
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOTX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OTX2
Function Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Cellular Location Nucleus.
Tissue Location Expressed in brain.
Research Areas
Citations (0)

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References

1. Hum Mutat. 2008 Nov;29(11):E278-83. 2. Cancer Res. 2010 Jan 1;70(1):181-91.

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$ 385.00
Cat# AO1568a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
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